Figure 2. The role of TGR5 in TDCA-induced DNA damage.

(A) Bile acid TDCA significantly increased the tail moment. Knockdown of TGR5 significantly decreased the tail moment in response to TDCA treatment in FLO-1 cells (N = 134-253 cells of 3 experiments). (B) Representative images of the Comet Assay at 4X magnification in FLO-1 cells transfected with pcDNA3.1 or TGR5 plasmid with or without TDCA treatment (10⁻¹¹ M, 24 hours). C) Overexpression of TGR5 significantly increased the tail moment in response to TDCA treatment in FLO-1 cells (N = 190–263 cells of 3 experiments). These data suggest that TGR5 may mediate bile acid-induced DNA damage. ANOVA **P < 0.0001, compared with Control siRNA group or pCDNA group; ^#P < 0.0001, compared with Control siRNA group plus TDCA group; ^##P < 0.05, compared with Control pCDNA group plus TDCA group.