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. 2016 Aug 11;6:31509. doi: 10.1038/srep31509

Figure 2. Involvement of RSK1 activation in the P4-induced migration enhancement in T47D cells.

Figure 2

(A) P4 (50 nM) treatment increased the levels of RSK1, p27 and p27pT198 protein in T47D cells. Pre-treatment with a RSK1 inhibitor, BI-D1870 (31 nM), prevented P4-induced increases in p27 phosphorylation at T198 (B), membrane translocation of RhoA (C), and migration enhancement (D). (E) Pre-treatment of the cell with RSK1 antisense oligonucleotide prevented the P4-induced migration enhancement in T47D cells. For Western blot analyses, data are representative of 2 (A) or 3 (B,C) independent experiments with similar results. Values shown in parentheses represent the quantified results adjusted with G3PDH (A,B) or cadherin (C) and expressed as ratio over its own control. Caherin and G3PDH were used as a membrane and cytosolic protein marker, respectively, to confirm the purities of isolation and to verify equivalent sample loading. In (B–E), values represent the means±s.e.mean. (n = 3). *P < 0.05 different from control group. #P < 0.05 different from P4-treated group. AS, antisense; BI, BI-D1870; Con, control; SC, scramble.