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. 2016 Aug 11;6:31509. doi: 10.1038/srep31509

Figure 4. Roles of AKT and ERK1/2 activation in the P4-induced migration enhancement in T47D cells.

Figure 4

(A) Treatment with P4 (50 nM) for 15 min increased the levels of p-RSK1 and p27pT198, and these effects were abolished by pre-treatment of the cell with dn-AKT, but not U0126 (1 μM). (B) Treatment with P4 (50 nM) for 6 h increased the levels of RSK1 and p27 protein, and these effects were abolished by pre-treatment of the cell with U0126, but not dn-AKT. Treatment with P4 (50 nM) for 20 min induced IκBα activation (C) and NFκB nuclear translocation (D), and these effects were abolished by pre-treatment of the cell with U0126, but not dn-AKT. Western blot data are representative of 2 (B–D) or 3 (A) independent experiments with similar results. Values shown in parentheses represent the quantified results adjusted with their own total protein (A,C), G3PDH (B), or PARP (D). PARP and G3PDH were used as a nucleus and cytosolic protein marker, respectively, to confirm the purities of isolation and to verify equivalent sample loading. The P4 (50 nM)-induced migration enhancement was abolished by pre-transfection of cells with dn-AKT (E) or partially reduced by pre-treatment with 1 μM of U0126 (F) in T47D cells. In (A,E,F), values represent the means±s.e.mean. (n = 3). *P < 0.05 different from control group. #P < 0.05 different from P4-treated group. Con, control; dn-AKT, dominant negative AKT.