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. 2016 Aug 11;6:31509. doi: 10.1038/srep31509

Figure 6. Effects of P4 on the activation of the cSrc/AKT/RSK1 signaling pathway and p27 phosphorylation at T198 in MCF-7 cells.

Figure 6

(A) Treatment with P4 (50 nM) for 5 min induced cSrc activation in MCF-7 cells. (B) Pre-treatment with PP2 (100 nM) abolished the P4-induced activations in AKT and ERK1/2. (C) Pre-treatment with wortaminnin (100 nM) abolished P4-induced increases of the levels of p-RSK1 and p-p27T198 protein. (D) The P4-induced phosphorylation of p27 at T198 in MCF-7 was abolished by pre-treatment with BI-D1870. Western blot data are representative of 2 independent experiments with similar results. Values shown in parentheses represent the quantified results adjusted with G3PDH. Con, control; BI, BI-D1870; WM, wortmannin.