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. 2016 Aug 11;6:31577. doi: 10.1038/srep31577

Figure 1. Pep19-2.5 and Pep19-4LF show low cytotoxicity in primary human keratinocytes and fibroblasts.

Figure 1

(A–C,E) Keratinocytes and (D,E) fibroblasts were stimulated for 24 h with different concentrations of Pep19-2.5 and Pep19-4LF and analysed by cell viability assays. (A,D) Dose-response curves were obtained by MTT assay. Data are mean ± SD (n = 3-4). (B,E) IL-8 release into medium was quantified by ELISA. Data are mean + SD (n = 3-6). (C) Annexin V-FITC and PI staining followed by flow cytometry analysis. Double positive cells are displayed as fold change compared to control. Data are mean + SD (n = 3-4).