Figure 6. Peptide-induced keratinocyte migration depends on purinergic receptors and metalloproteases.

(A) HaCaT cells were scratched and stimulated with Pep19-2.5 in the presence or absence of the inhibitors AG1478 (50 nM), marimastat (10 μM) and PPADS (50 μM). TGF-β₁ served as positive control. Images were taken directly after scratching (0 h) and after 20 h and are representative of three independent experiments. (B) HaCaT cells were stimulated with Pep19-2.5 and Pep19-4LF in the presence or absence of the inhibitors AG1478 (50 nM), marimastat (10 μM) and PPADS (50 μM). After 24 h EdU incorporation was quantified. Data are normalised to unstimulated cells (assigned as 1.0). Cells incubated with growth medium served as positive control. Data are mean + SD (n = 3).