Figure 3.

The radial distribution of coniferin evaluated by (b) HPLC and (c) cryo-TOF-SIMS. Preparation procedure of tangential sections for HPLC measurements is shown in (a). In (b), serial tangential sections of 100-μm thickness were used and the position of cambial zone corresponding to the section numbers 9 and 10 was determined by the dry weight of the sections as shown in Supplementary Fig. 9. The means and standard deviations for each section in (b) were obtained from three sets of measurements using the different sample blocks cut from the same disk. In (c), the m/z 180 ion count from bark to xylem was used and arrows at the distance 1100 μm indicate the position of the cambial zone.