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. 2016 Aug 11;6:30583. doi: 10.1038/srep30583

Figure 5. TRPV4 regulates the outflow facility in 3D pTM nanoscaffold models of conventional outflow together with remodeling of the cytoskeleton and extracellular matrix.

Figure 5

pTM-seeded SU-8 scaffolds. (a) Representative IHC images of F-actin and fibronectin following 24 hrs of perfusion. Scale bars = 30 μm. (b) Quantification of F-actin signals from (a). (c) Quantification of fibronectin signals from (a). (d) HC-06 (1 μM) increases the outflow the facility in 3D pTM cultures, whereas GSK101 (25 nM) non-significantly attenuates it (N = 5 experiments from 3 donors). (e) Relative to the vehicle control, HC-06 lowers perfusate pressure, whereas GSK101 has the opposite effect (N = 5 experiments from 3 donors).