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. 2015 Oct 23;2(1):92–109. doi: 10.1016/j.jcmgh.2015.09.007

Figure 1.

Figure 1

Transplant of enteric nervous system (ENS) cells to autologous colon explants from Hirschsprung disease (HSCR) patients. ENS cells from the proximal region of resected colon were sorted by flow cytometry after immunolabeling for p75. Aneuronal smooth muscle fragments were obtained from the distal region. Cocultures of these were established to test the ENS-forming ability of p75+ cells in autologous HSCR patient aneuronal colon muscle. CM, circular muscle; EdU, 5-ethynyl-2′-deoxyuridine; eGFP, enhanced green fluorescent protein; LM, longitudinal muscle; M, mucosa; MTR, MitoTracker Red.