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. 2015 Oct 23;2(1):92–109. doi: 10.1016/j.jcmgh.2015.09.007

Figure 3.

Figure 3

Characterization of human enteric nervous system (ENS) in vivo and in vitro. (A) The proximal resected Hirschsprung disease (HSCR) colon contained myenteric ganglia, positive for p75 and the neuron marker Hu (open arrowhead) and glia/progenitor marker SOX10 (closed arrowhead). (B) Near the enteric ganglia were putative progenitor cells, identified by SOX10 and absence of the glia marker GFAP (glial fibrillary acidic protein) (closed arrowhead). (C) Dissociated colon cells on fibronectin grew initially as a monolayer. (D) Dissociated colon cells in vitro without fibronectin mainly formed spheres. (E) Immunolabeling of NC cell-surface antigens p75 and HNK1 (human natural killer-1) and transcription factor SOX10 reveal ENS cells within colon monolayer cultures. (F) Glia cells (SOX10+/S100β+) and neurons (SOX10−/HU+) occur in monolayer cells. Hst, Hoechst nuclear stain. Images acquired using confocal microscopy. Data derived from six HSCR patient samples.