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. 2015 Oct 23;2(1):92–109. doi: 10.1016/j.jcmgh.2015.09.007

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Human enteric nervous system (ENS)–derived cells proliferate in vitro. (A) Incorporation of ethynyl-2′-deoxyuridine (EdU) (white arrowheads) into SOX10⁺ ENS cells with and without CHIR-99021. (B) Proliferation of SOX10⁺ cells is significantly increased by CHIR-99021, expressed as a ratio of EdU⁺/SOX⁺ cells to total SOX10⁺ cells. (C) Proliferation occurs in putative progenitor cells (EdU⁺/SOX10⁺/S100β-ve; white arrowheads), but was not detected in neurons (HU⁺/SOX10-ve; open arrowhead) during the culture period. Images acquired using confocal microscopy. Data derived from two Hirschsprung disease (HSCR) patient samples.