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. 2015 Oct 23;2(1):92–109. doi: 10.1016/j.jcmgh.2015.09.007

Figure 7.

Figure 7

Mouse enteric nervous system (ENS) cells colonize Hirschsprung disease (HSCR) aneuronal colonic muscle. (A) EdnrbKik+ mouse ENS cell spheres (white arrowhead) were combined with human HSCR muscle tissue (day 0) by positioning the sphere between the longitudinal muscle (LM) and circular muscle (CM) layers. The inset image shows that after 7 days of culture in vitro, Kikume cells have spread throughout the explant. (B) EdnrbKik+ ENC cells formed ganglion-like clusters with neurons (Hu+) and glial or progenitor cells (SOX10+). These ganglion-like clusters contained nitric oxide synthase positive (NOS+) neural subtypes (C) and also glia (GFAP+, glial fibrillary acidic protein positive) (D). (E) Proliferating EdnrbKik+ ENC cells differentiated into neurons (EdU+/Hu+) (white arrowheads) within human HSCR muscle. (F) Combination cultures of EdnrbKik cells with HSCR colon tissue for 5 days shows synaptophysin immunoreactivity closely associated with Kikume graft–derived nerve fibers. Results were from 8 explants from two HSCR patients. Images were acquired by confocal microscopy.