Figure 8.

Autologous transplant of human p75⁺/eGFP enteric nervous system (ENS)-derived cells in Hirschsprung disease (HSCR) muscle. (A) p75⁺/eGFP ENS aggregate combined with HSCR muscle (day 0) and after the culture period (day 5). During this period enhanced green fluorescent protein (eGFP) cells have spread throughout the muscle explant. Most eGFP⁺ cells spread on the circular muscle (CM) and at the interface (dotted line) with the longitudinal muscle (LM); this is shown (white arrowhead) in the side view of the lower explant at day 5. (B) Confocal whole-mount immunolabeling revealed that the majority of eGFP cells maintained expression of SOX10 and these cells were found extensively over muscle tissue (white arrowheads). SOX10⁺ cells not expressing eGFP were also detected and are likely of glial origin, which reside in the aneuronal tissue (see Fig. 2).