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. 2015 Oct 23;2(1):92–109. doi: 10.1016/j.jcmgh.2015.09.007

Figure 9.

Figure 9

Autologous transplant of human p75+/MTR (MitoTracker Red) enteric nervous system (ENS)–derived cells in Hirschsprung disease (HSCR) muscle. (A) p75+ ENS aggregate prestained with MTR before implantation into HSCR muscle. The insert shows MTR labeling within the cytoplasm of SOX10+ cells. (B) Implantation of aggregate with HSCR muscle shows distribution of MTR+/p75+ cells from initial graft site (indicated by white dotted circle) after 5 days. (C, D) Ganglion-like structures originating from MTR+ aggregates contain SOX10+ and p75+ cells, glia markers (S100β+) and nerve fibers (TUJ1+). (E) Proliferating human p75+ ENS cells differentiate into neurons (EdU+/Hu+) within HSCR muscle (white arrowhead). (F) Neuronal nitric oxide synthase (nNOS) immunoreactivity in MTR-labeled cells indicates neuronal differentiation appropriate of an ENS neuron type from human p75+ ENS cells. Images acquired using confocal microscopy.