Table 2.
Oligonucleotide Primers Used in Quantitative Reverse-Transcription Polymerase Chain Reaction Amplification of Human Genes
| Gene | Sequence | Amplicon Size (bp) | Primer Design | Gene Role |
|---|---|---|---|---|
| SOX10 | Sense: GCTGCTGAACGAAAGTGACAAG Antisense: TCTTGTAGTGGGCCTGGATG |
197 | Spans exons 3–4 (intron 3) | Early NC development and marks migrating ENC cells, Glia |
| AP2 | Sense: ACTCGGAGACCTCTCGATCC Antisense: GGACACGGGGCCTTTCTTAAT |
115 | Spans exons 2–4 (introns 2 and 3) | Specifier gene for early induction of ENC fate |
| FOXD3 | Sense: CCCAAGAACAGCCTAGTGAAGC Antisense: TTCTCCCTGTAGTAGGGGAAGC |
140 | Monoexonic transcript (non-intron spanning) | Maintenance of ENC progenitor |
| P75 | Sense: ATCCCTGTCTATTGCTCCATCC Antisense: TGTGGAGTTTTTCTCCCTCTGG |
154 | Spans exons 4–5 (intron 4) | Nerve growth factor receptor, labels all ENS |
| RPL32 | Sense: CATCTCCTTCTCGGCATCA Antisense: ACCCTGTTGTCAATGCCTC |
153 | Spans exons 1–3 (introns 1 and 2) | Non ENS, gene for qRT-PCR normalization |
ENC, enteric neural crest; ENS, enteric nervous system; NC, neural crest; qRT-PCR, quantitative reverse-transcription polymerase chain reaction.