Table 3.
Antibodies and Probes Used to Label Enteric Nervous System and Muscle Cells
| Cell Target | Identity | Type | Source | Product No. | Research Resource Identifiers (RRID) |
|---|---|---|---|---|---|
| NC cell surface | p75 | Rabbit IgG | Promega | G3231 | AB_430853 |
| NC cell surface/FACS | p75: Alexa 647, clone c40–1457 | Mouse IgG | BD Pharmingen | 560326 | AB_2033986 |
| NC cell surface | HNK1 | Mouse IgM | MCRI | NA | NA |
| NC/glial cell nucleus | SOX10 | Goat IgG | R&D Systems | AF2864 | AB_442208 |
| Glial cytoplasm | GFAP | Rabbit IgG | Dako, Denmark | Z0334 | AB_2314535 |
| Glial cytoplasm | S100β, clone SH-B1 | Mouse IgG | Sigma-Aldrich, Australia | S2532 | AB_477499 |
| Neuron nucleus/cytoplasm | Hu | Human IgG | Vanda Lennon, Rochester, MN | NA | NA |
| Nitrergic neuron | nNOS | Sheep IgG | EMSON | H212 | AB_2314957 |
| Nerve fiber | Neuronal class III β-tubulin clone (TUJ1) | Mouse IgG | Biolegend/Covance | MMS-435P | AB_2315514 |
| Smooth muscle actin | SMA, clone SH-B1 | Mouse IgG | Sigma-Aldrich, Australia | A2547 | AB_476701 |
| DNA | Hoechst 33342 | NA | Life Technologies | H3570 | NA |
| DNA/dead cells | Propidium iodide | NA | Sigma-Aldrich Australia | P4170 | NA |
| Mitochondria | MitoTracker CMXRos | NA | Life Technologies | M7512 | NA |
| Secondary antibodies and probes | |||||
| Human IgG | Texas Red | Donkey IgG | Jackson ImmunoResearch | 709 075 098 | AB_2532064 |
| Mouse IgM | Texas Red | Donkey IgG | Jackson ImmunoResearch | 715 065 140 | AB_2340783 |
| Mouse IgG | Alexa 594 | Donkey IgG | Life Technologies | A21203 | AB_10563558 |
| Mouse IgG | Alexa 647 | Goat IgG | Life Technologies | A21235 | AB_10562370 |
| Goat IgG | Alexa 488 | Donkey IgG | Life Technologies | A11015 | AB_10561557 |
| Goat IgG | Alexa 594 | Donkey IgG | Life Technologies | A11058 | AB_142540 |
| Goat IgG | Biotin | Donkey IgG | Jackson ImmunoResearch | 705 065 147 | AB_2340397 |
| Biotin | AMCA | Streptavidin | Jackson ImmunoResearch | 016 150 084 | AB_2337243 |
AMCA, methyl N-(4′-(9-acridinylamino)-phenyl) carbamate hydrochloride; GFAP, glial fibrillary acidic protein; HNK1, human natural killer-1; NA, not applicable; NC, neural crest; nNOS, neuronal nitric oxide synthase; SMA, smooth muscle actin; SOX10, sex-determining region Y–box 10.