Table 2.
Taxonomic characterization of bacterial isolates SAT4.
| Test analysis | Types | Features | Result |
|---|---|---|---|
| Microscopic examination | Gram’s staining | + | |
| Shape | Rods arranged in pairs | ||
| Morphological characteristics | Spore formation | + | |
| Motility | + | ||
| Macroscopic appearance: colony morphology | Color | White to off | |
| Elevation | Flat | ||
| Colony shape | Circular | ||
| Margins | Entire | ||
| Biochemical characteristicsc |
API 50CHB Tests that were positive fora | ||
| GLY GLU FRU MNE MAN SOR MDG NAG AMY ARB ESC SAL CEL SAC TRE LFUC + + + + + + + + + + + + + + + + | |||
| API 20E Tests that were positive forb | |||
| ONPG CIT GEL INO RHA ARA + + + + + + | |||
| Significant Taxon 98.4 % Geobacillus pallidus | |||
| Molecular | Universal primers F27 and R907 | ||
| Characteristics | 100% homology with Aeribacillus pallidus at NCBI server (accession number: JN986827) previously known as Geobacillus pallidus[21] | ||
Indications: + sign indicates the positive reaction (color change), GLY: glycerol; GLU: d-glucose; FRU: d-fructose; MNE: d-mannose; MAN: d-mannitol; SOR, MDG, NAG: N-acetylglucosamine; AMY: amygdalin; ARB: arbutin; ESC: esculin ferric citrate; SAL: salicin; CEL: d-cellobiose; SAC: d-sucrose; TRE: trehalose; LFUC: l-fucose.
Only the tests with a positive result are included here (remaining tests were negative).
Only the tests with a positive result are included here (remaining tests were negative). ONPG: 2-nitrophenyl-ß-d-galactopyranoside; CIT: trisodium citrate; GEL: gelatin; INO: inositol; RHA: l-rhamnose; ARA: l-arabinose.
Biochemical tests: API kit system with percentage of positive tests after 24 h of incubation.