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. Author manuscript; available in PMC: 2016 Aug 11.
Published in final edited form as: J Alzheimers Dis. 2016 Apr 8;52(4):1263–1275. doi: 10.3233/JAD-160033

Figure 3. Immunoprecipitation of molecules cross-linked to TTR in 7PA2 (APPVal717Phe) cells transfected with a wt hu TTR plasmid.

Figure 3

The 7PA2 cells containing the two constructs were incubated overnight with 1µM DAPT to decrease digestion of CTF then lysed and the lysates analyzed by western blotting. Panel A shows that the anti-TTR antibody (DAKO,linked to Dynabeads) pulls down complexes that had been cross-linked intracellularly by the membrane permeable cross-linker DSP. The complexes contained TTR, CTF, APP proteins only from the 7PA2 cells expressing APPVal717Phe transfected with the pcDNA-TTR construct, not from cells transfected with the control LacZ construct. Similar results were seen in 7WD10 cells which express wt AβPP (not shown). Panel B shows the lysate from which the immunoprecipitates were prepared.