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. Author manuscript; available in PMC: 2016 Aug 11.
Published in final edited form as: J Alzheimers Dis. 2016 Apr 8;52(4):1263–1275. doi: 10.3233/JAD-160033

Figure 5. CHO cells expressing APP, i.e. 7PA2 (APPVal717Phe), 7WD10 (wt APP), and SH-SY5Y cells transfected with pcDNA-LacZ and pcDNA-TTR.

Figure 5

Panel A shows that the amount of Aβ protein as determined by ELISA is reduced in all the cell lines that also express human TTR. Panel B shows that while the amount of Aβ released into the media by the TTR-transfected 7PA2 cells was diminished, the amounts of Aβ in the cell lysates were similar in the presence and absence of TTR. The experiments with the 7PA2 and 7WD10 cells were repeated three times. The SH-SY5Y experiment was done only once. Panel C shows that the amounts of secreted sAPPβ (the product of BACE cleavage of AβPP) were similar, suggesting that the effects of TTR were downstream of the generation of sAPPβ and C99 by BACE1.