Table 2. In vitro functional validation of candidate invasion driver genes.
Twenty-one candidate invasion driver genes, which were not previously implicated in CRC (black type), in addition to one positive control (red type) and 5 negative control (blue type) genes, were assayed for their ability to increase cell proliferation by WST-1 dye staining, or migration and invasion through matrigel in Boyden chambers, in hCEC or SW480 cells, following siRNA knockdown.
| Gene | hCEC | SW480 | ||||
|---|---|---|---|---|---|---|
| proliferation | migration | invasion | proliferation | migration | invasion | |
| LATS2 | + | + | + | |||
| AFF3 | ||||||
| ANTXR1 | -- | -- | ||||
| ASXL2 | + | ++ | ||||
| CAMTA1 | + | ++ | + | ++ | ||
| CSTF3 | ||||||
| DBF4 | ||||||
| DDX20 | + | + | + | |||
| DENND2C | ||||||
| DMXL2 | ||||||
| FGF13 | ||||||
| FXR1 | + | + | + | + | ||
| GRM8 | ||||||
| KDM2B | + | |||||
| LPP | ||||||
| MITF | + | + | + | |||
| PAX7 | + | + | ++ | + | ||
| PDE4DIP | -- | -- | ||||
| PRKG1 | + | |||||
| RPAP1 | + | |||||
| SEMA6D | ||||||
| SUPT3H | -- -- | -- | -- -- | -- | ||
| CCDC113 | ||||||
| KLHL10 | ||||||
| POMZP3 | ||||||
| RNPC3 | ||||||
| SMTNL2 | ||||||
+two-fold increase
++three-fold increase
--two-fold decrease
-- --three-fold decrease, compared to APC shRNA-expressing hCECs or parental SW480 cells transduced with control oligos.