Figure 3.

ITC Analysis of Peptide-CaM Interactions.

For all experiments, calorimetric titrations (top panels) and the least-squares fitted model of binding are shown (bottom panels). Calculated K_d values are shown for each modeled binding site.

(A) Titration of 150 µM NT peptide into 16 µM CaM in the presence of 5 mM CaCl₂.

(B) Titration of 205 µM IQ peptide into 20 µM CaM in the presence of 5 mM CaCl₂.

(C) Titration of 150 µM CT peptide into 16 µM CaM in the presence of 5 mM CaCl₂.

(D) Titration of 138 µM IQ peptide into 20 µM CaM in the presence of 1 mM EGTA.