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The I572D/Q573A Mutation Disrupts CaM Binding to the CNGC12 IQ Motif.
ND-PAGE was performed with IQ (wild-type sequence) or IQmut peptide (bearing the I672D/Q573A mutation). Closed and open arrows indicate the migration of free or peptide-bound CaM, respectively, while the two distinct bands formed by the IQ-Ca2+/CaM complex are numbered 1 and 2.
