Figure 7.

The IQ Motif Is Required for CNGC11/12-Induced PCD.

(A) N. benthamiana leaf 4 dpi (bar = 1 cm).

(B) RT-PCR of cDNA from N. benthamiana leaves 24 hpi expressing CNGC11/12 or site-directed mutant constructs. Nb-ACTIN was used as a loading control (26 cycles).

(C) GFP confocal microscopy of N. benthamiana leaves 30 hpi (bar = 10 µm) and Trypan blue (TB) staining for cell death in N. benthamiana leaves 3 dpi (bar = 0.1 mm). E.V., empty vector (GFP); 11/12, CNGC11/12; 11/12-IQ^mut, CNGC11/12^I564D/Q565A; 11/12-CT^mut, CNGC11/12^V599E.