Figure 10.

SDG8 and SDG25 Regulate Lipid Accumulation and Cuticle Permeability.

(A) and (B) Lipid accumulation in root meristem regions of sdg, ccr2, and cer3 mutants.

(A) Nile red staining of polar and nonpolar lipids.

(B) Quantification of the fluorescence intensities of polar and nonpolar lipids in the root meristem regions.

(C) and (D) Lipid accumulation in root mature regions of sdg, ccr2, and cer3 mutants. Nile red staining of polar and nonpolar lipids (C) and quantification of the fluorescence intensities of polar and nonpolar lipids in the root mature regions (D). The mean values followed by different letters indicate significant differences (P < 0.05, Student’s t test). Top panels, polar lipids; middle panels, nonpolar lipids; bottom panels, merged image from the other two panels. Bars = 100 μm. The lipid levels were visualized by Nile red staining of root tissues as described in Methods. In (B) and (D), the fluorescence intensities were quantified with the ImageJ software (http://rsb.info.nih.gov/ij/download.html). Values represent mean of 10 measurements ±sd.

(E) and (F) Increased cuticle permeability in sdg, ccr2, and cer3 mutants. Leaves from 4- to 5-week-old plants were incubated in 0.05% toluidine blue solution for 30 min. The experiments were performed three times with similar results. Wt, wild type; sdg8, sdg8-2; sdg25, sdg25-1. Bars = 1 cm.