Figure 5.

Arabidopsis DDL Is Not Involved in Plastid Division.

(A) Schematic representation of the At-DDL locus in the Arabidopsis genome. The T-DNA insertion positions in the SALK_092419C (5th intron, At-ddl-1) and Sail_906_E06 (11th exon, At-ddl-2) lines are marked with triangles. Black boxes and lines represent exons and introns, respectively. The locations of the primers (AtDdlF0 and AtDdlR0) used for RT-PCR are indicated.

(B) Genomic PCR data with a gene-specific primer (LP and RP) and a primer corresponding to the sequence of the T-DNA left border (LB).

(C) RT-PCR data derived using At-DDL primers are shown. The Arabidopsis ACTIN gene was used as an internal control.

(D) Protoplasts of mesophyll cells from wild-type, At-ddl-1, and At-ddl-2 plants. Bar = 10 µm.