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. 2016 Jun 17;28(7):1581–1601. doi: 10.1105/tpc.16.00286

Figure 4.

Figure 4.

SMXL7 Responds to SL Treatment in Leaves and Stems.

Red signal is chloroplast fluorescence. Yellow signal is SMXL7-YFP/VENUS.

(A) to (H) Response of SMXL7 variants in leaf petioles to 20 min treatment with 5 μM rac-GR24: SMXL7-VENUS in the Col-0 ([A] and [B]) and max2-1 ([C] and [D]) backgrounds; SMXL7d53-VENUS in Col-0 ([E] and [F]) and SMXL7d53-YFP in Col-0 ([G] and [H]). Expression from the SMXL7 promoter ([A] to [F]) or 35S promoter ([G] and [H]).

(I) Very low levels of SMXL7-VENUS in longitudinal hand sections through Col-0 stems.

(J) to (P) Response of SMXL7 variants expressed in longitudinal hand sections of stems to treatment with 5 μM rac-GR24 (treatment time indicated in panel); SMXL7-VENUS in smxl6-4 smxl7-3 smxl8-1 max2-1 (s678m2) (J) and max1-1 ([K] and [L]) backgrounds; SMXL7d53-VENUS in Col-0 ([M] and [N]); SMXL7d53-YFP in Col-0 (O) and SMXL7ΔP-loop-YFP in Col-0 (P). Expression from the SMXL7 promoter ([I] to [N]) or 35S promoter ([O] and [P]). White arrows indicate SMXL7 expressing nuclei before and after treatment with rac-GR24.