Table 1. Arabidopsis Transgenic Lines.
| Background | Construct | Copy No. | GR24 Response | Total T1 Lines | T1 Lines with Representative Phenotype |
|---|---|---|---|---|---|
| Col-0 | SMXL7pro:SMXL7-VENUS | 4 | Y | 4 | 4 |
| s678 max2 | SMXL7pro:SMXL7-VENUS | 2 | Y | 5 | 5 |
| max2-1 | SMXL7pro:SMXL7-VENUS | 4 | N | 5 | 5 |
| max1-1 | SMXL7pro:SMXL7-VENUS | 4 | Y | Crossed | |
| Col-0 | SMXL7pro:SMXLd53-VENUS | 4 | N | 6 | 6 |
| s678 max2 | SMXL7pro:SMXL7d53-VENUS | 2 | N | 3 | 3 |
| s678 | SMXL7pro:SMXL7d53-VENUS | 2 | N | Crossed | |
| Col-0 | SMXL7pro:SMXL7ΔP-loop-VENUS | 4 | N | 4 | 3 |
| s678 max2 | SMXL7pro:SMXL7ΔEAR-VENUS | 2 | Y | 6 | 5 |
| s678 max2 | SMXL7pro:SMXL7alaEAR-VENUS | 2 | N | 4 | 4 |
| s678 max2 | SMXL7pro:SMXL7ΔP-loop,ΔEAR-VENUS | 2 | N | 4 | 3 |
| Col-0 | SMXL7pro:SMXL7d53,alaEAR-VENUS | 4 | N | 3 | 3 |
| s678 | SMXL7pro:SMXL7d53,alaEAR-VENUS | 2 | N | 2 | 2 |
| Col-0 | 35Spro:SMXL7-YFP | 4 | Y | 2 | 2 |
| s678 max2 | 35Spro:SMXL7ΔNLS-YFP | 2 | N | 5 | 5 |
| Col-0 | 35Spro:SMXL7d53-YFP | 4 | N | 6 | 2 |
| Col-0 | 35Spro:SMXL7ΔP-loop-YFP | 4 | N | 5 | 2 |
| s678 max2 | 35Spro:SMXL7ΔEAR-YFP | 2 | N | 5 | 3 |
| s678 max2 | 35Spro:SMXL7alaEAR-YFP | 2 | N | 5 | 2 |
| Col-0 | 35Spro:SMXL7ΔP-loop,ΔEAR-YFP | 4 | N | 3 | 3 |
List of stably transformed SMXL7 protein fusion lines in Arabidopsis used in this study. Constructs were transformed into the indicated genetic backgrounds; the total number of T1 lines analyzed is indicated, along with the number of lines showing the phenotypes discussed here. The copy number of SMXL7 in these lines and whether the transgenic whether the transgenic SMXL7 protein can be degraded in response to rac-GR24 are also indicated.