Figure 2.

Characterization of EMS Mutations in 252 Sorghum Samples.

(A) Mutation density (SNP/Mb) in each sample.

(B) SIFT score distribution of the EMS mutations. Mutations with SIFT scores <0.05 were considered to be deleterious to protein structure or gene function.

(C) Distribution of large-effect mutations in each sample. Large-effect mutations include stop-gain mutations, nonsynonymous coding mutations with a SIFT score <0.05, and mutation at splice acceptor (two bases before exon start, except for the first exon) or donor sites (two bases after coding exon end, except for the last exon).

(D) Distribution of the number of large-effect mutations in each gene.