Figure 3.

Phenotype of Bloomless Mutants and Genetic Evidence.

(A) Characterization of bloomless mutants. Phenotype shows the presence or absence of epicuticular wax in stems of wild-type and mutant lines. The chromatogram from fatty acid analyses confirmed that C30 fatty acid, which is predominant in the wild type, was significantly reduced in bloomless mutants.

(B) Scatterplots of endpoint genotyping results from an F2 population of ARS 20 for SbCER5 and SbCER6.

(C) Genotype and phenotype data of F2 individual plants from ARS20. +/+, Wild type; cer5/+ and cer6/+, heterozygous mutants; cer5/- and cer6/-, homozygous mutants.