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. 2016 Apr 20;10(2):103–111. doi: 10.1080/19336896.2016.1141858

FIGURE 2.

FIGURE 2.

Hsp31 cannot resolve the Sup35 amyloids. [PSI+] cells were transferred with pAG415-GPD-Hsp31-DsRed, p2HG Hsp104 and their corresponding empty vectors. Cultures were incubated at 30°C for 16 hrs before plating on ¼ YPD medium to develop colony color. Change from white to red color phenotype demonstrated curing. Hsp104 is able to cure the [PSI+] phenotype as seen by the red colonies. Hsp31 expression under these conditions can transiently reduce prion aggregation as shown by semi-denaturing gels and microscopy but is not able to cure prions.