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. 2016 Jun 8;203(4):1601–1611. doi: 10.1534/genetics.115.184879

Figure 5.

Figure 5

Senescence characteristics of AB-C1 cancer cells. (A) Morphological comparison of 23116 MT vs. AB-C1 cells (×20 magnification). (B) Representative images of indicated cells stained for senescence-associated β-galactosidase activity. (C) Average percentages of positively stained cells (blue color) calculated from four technical replicates. (D) qRT-PCR validation of senescence-associated genes. (E) qRT-PCR assay comparing relative transcript levels of p21 in AB-C1 vs. 23116 MT cells. Significant differences were calculated using a two-tailed Student’s t-test (* P < 0.05; ** P < 0.001). Values are relative to untransduced parental cell line 23116 MT.