Figure 6.

Overexpression of Arid1a in the TRP53-deficient MCN1 has no effect on proliferation rate or tumor growth. Arid1a expression levels were quantified by (A) qRT-PCR and (B) immunoblotting in MCN1 MT cell line and its transduced counterpart cell line (MCN1-C6). Significant differences were calculated using a two-tailed Student's t-test (** P < 0.001). Values are relative to untransduced parental cell line MCN1. (C) EdU incorporation assays of MCN1 vs. MCN1-C6. Error bars signify the mean ± SEM of three experimental replicates, with >1000 cells counted per sample for each replicate. (D) Representative images of tumors arising from transplantation of MCN1 (MT-R) and MCN1-C6 (MT-L) cells into cleared fad pads of recipient syngeneic FVB/N recipient females. MT-R and MT-L refer to right and left sides of mouse, respectively (see Materials and Methods). The right panel plots individual weights of tumors (n = 10 mice, 20 tumors).