Figure 3.
HACK can be applied to convert commonly-used GAL4 lines at various genomic locations. (A) Cytological locations of QF2G4H and GAL4 lines used in this study. The GAL4 lines are distributed across the second and third chromosomes. (B–C) Examples of QF2G4H lines generated by the HACK method using GAL4 lines with (B) broad (e.g., pan-glial) and (C) defined (e.g., class IV multidendritic neurons) expression patterns. Purple, nc82 antibody staining; Green, GFP antibody staining. Bar, 100 µm (brain and ventral nerve cord).