ChChd3 depletion causes tissue undergrowth. (A–C) Adult female eyes expressing the following transgenes under the control of ey-Gal4: (A) control, (B) UAS-ChChd3 RNAi I (ChChd3-IR1), and (C) UAS-ChChd3 RNAi II (ChChd3 IR2). (D) Quantification of eye size of the indicated genotypes. n = 10 for each genotype. (E–G′′′) ChChd3 expression in wing imaginal discs expressing UAS-GFP (E–E′′′), UAS-ChChd3 RNAi I (F–F′′′), and UAS-ChChd3 RNAi II (G–G′′′) under the control of en-Gal4. The en-expressing domain was marked by GFP. ChChd3 protein was effectively knocked down by RNAi. Bar, 100 μm.