Figure 2.

Loss of function of ChChd3 results in growth defects. (A) Schematic representation of the ChChd3 locus. The deletion in ChChd3^D1 mutant allele is indicated by the bracketed area. (B) Western blot on second instar larval extracts from wild-type and ChChd3^D1 mutant animals. Lysates were probed with anti-ChChd3 and anti-tubulin. (C) Wild-type control and mutant larvae homozygotic for ChChd3^D1 or transheterozygous for ChChd3^D1 and a deficiency after 5 days of growth. D1 denotes the ChChd3^D1 mutant allele. Df denotes the Df(3R)BSC749 deficiency line removing the ChChd3 locus. (D–D′′) Wing imaginal disc with control mitotic clones (lack of β-Gal, black area) and their corresponding twin spots (two copies of β-Gal, brighter area) of similar size. (E–E′′) Wing imaginal disc with ChChd3^D1 homozygous mutant clones (lack of β-Gal) that are smaller than their corresponding twin spots (two copies of β-Gal). (F) Measurements of clone area for 25 pairs of control clones and their sister twin spots. (G) Measurements of clone area for 25 pairs of ChChd3^D1 homozygous mutant clones and their sister twin spots. (H–I′′) Wing imaginal discs containing control (H–H′′) or ChChd3^D1 homozygous mutant (I-I′′) clones stained with anti-β-Gal and anti-PH3 antibodies. ChChd3 mutant clone had reduced number of cells positive for PH3 staining. (J–K′′) Wing imaginal discs containing control (J–J′′) or ChChd3^D1 homozygous mutant (K–K′′) clones stained with anti-β-Gal and labeled with EdU. ChChd3 mutant clone had reduced number of cells positive for EdU labeling. Asterisks indicate the clone area. Bars, 100 μm.