Figure 5.
Loss of ChChd3 increases Hippo pathway target gene expression. (A–A′′′) Expression pattern of the Diap1-lacZ reporter gene in a control wing imaginal disc expressing the UAS-GFP transgene with en-Gal4. (B–B′′′) Knockdown of ChChd3 by RNAi with en-Gal4 increases the level of Diap1-lacZ expression in posterior cells. Discs (in A–B′′′) were stained with anti-β-Gal and anti-GFP. DAPI was used to label DNA. Posterior cells are marked by GFP. Dashed lines indicate the anterior/posterior compartment boundary. (C–E′′′) Upregulation of Diap1-lacZ (C–C′′′), ex-lacZ (D–D′′′), or CycE-lacZ (E–E′′′) expression in ChChd3D1 homozygous mutant clones in wing imaginal discs. Discs (in C–E′′′) were stained with anti-β-Gal and anti-RFP. DAPI was used to label DNA. Mutant clones are marked by lack of RFP and their corresponding twin spots are marked by two copies of RFP. Dashed lines indicate the clone outline. Bars, 100 μm in A and D; 50 μm in C.