Figure 6.

Yki is required for Hippo target gene expression and cell proliferation in ChChd3 mutant clones. (A–A′′′) Wing imaginal disc containing ChChd3^D1 mutant MARCM clones. (B–B′′′) Wing imaginal disc containing ChChd3^D1 mutant clones with overexpression of UAS-yki-RNAi. Note overexpression of UAS-yki-RNAi suppresses the upregulation of Diap1-lacZ expression in the ChChd3^D1 mutant background. Discs were stained with anti-GFP and anti-β-Gal. DAPI was used to label DNA. Mutant MARCM clones are marked by GFP and indicated by white asterisks. (C) Quantification of clone area of ChChd3^D1 mutant and ChChd3^D1 mutant with overexpression of UAS-yki-RNAi. n = 23 clones for each genotype. Bars, 50 μm.