Figure 7.
Loss of ChChd3 induces oxidative stress and activates JNK signaling. (A–A′′′) Upregulation of GstD1-GFP expression in ChChd3D1 homozygous mutant clones in a wing imaginal disc. Disc was stained with anti-GFP and anti-RFP. Mutant clones are marked by lack of RFP and outlined by the dashed line. (B–B′′′) Low expression of the puc-lacZ reporter gene in a control wing imaginal disc. (C–C′′′) Knockdown of ChChd3 by RNAi with ptc-Gal4 increases the level of puc-lacZ expression in a stripe of anterior compartment cells. ptc-expressing cells are marked by GFP and outlined by the dashed lines. Bars, 100 μm.