Figure 8.

Depletion of either Opa1 or Marf increases Hippo pathway target gene expression. (A–A′′′) Expression pattern of the Diap1-lacZ reporter gene in a control wing imaginal disc. (B–B′′′) RNAi knockdown of Opa1 increases the level of Diap1-lacZ expression. (C–C′′′) RNAi knockdown of Marf increases the level of Diap1-lacZ expression. Discs were stained with anti-β-Gal and anti-GFP. DAPI was used to label DNA. Posterior cells are marked by GFP. Dashed lines indicate the anterior/posterior compartment boundary. Bar, 100 μm.