Fig 7. Hepatocyte spheroids in honeycomb concave microwells exhibit greater liver-specific function than those in circular concave microwells.

(a) Analysis of metabolic functions of spheroids in the two types of microwells, as measured by the secretion of albumin. (b) Immunostaining of hepatocyte spheroids for albumin (primary hepatocytes; green in first row), DAPI (nuclei; blue), cytochrome P450 activity (CYP450; green in second row) and microfilaments (F-actin; red). (c) Quantified analysis of hepatocytes function test. Scale bars: 300 μm.