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. 2016 Aug 11;11(8):e0160943. doi: 10.1371/journal.pone.0160943

Fig 1. Quantitative RT-PCR (qRT-PCR) analysis of smeE, smeF, and smeZ transcripts.

Fig 1

The RNA was prepared from the exponentially growing cells, converted to cDNA by RT-PCR, and used as the template for quantitative reverse-transcriptase PCR (qRT-PCR). Data are the means of three independent experiments. Error bars indicate the standard deviation for three triplicate samples. *, p≤0.05 significance calculated by a Student’s t-test. (A) qRT-PCR analysis of smeE, smeF, and smeZ transcripts of strains KJ, KJΔRSy, KJΔRy, and KJΔYZ. (B) qRT-PCR analysis of smeE, smeF, and smeZ transcripts in the smeRy-complemented strains of KJΔRSy and KJΔRy.