Table 1. Primers used for relative qPCR to validate RNA-sequencing results.

Melt peaks listed were obtained on a BioRad CFX Connect Real-Time System and may vary by -/+ 1°C depending on the thermocycler.

Gene Symbol	Primer Sequence(5' to 3')	Reference Gene	Product Size (bp)	Melt Peak (°C)
ADIPOR1	FOR: TCCTGCCAGTAACAGGGAAG	Actin	168	86.5
	REV: AGGGGAAGTGTCAGTACCCG
AKT1	FOR: GTGGACCAACGTGAGGCTC	RPII	132	88.0
	REV: GAAGGTGCGTTCGATGACAG
BCAR3	FOR: CAGAAACATGCCGGTGAATCA	Actin, RPII	209	86.0–86.5
	REV: GTGGGGATTTGGAGTGGGG
B2M	FOR: GCTATCCAGCGTACTCCAAAG	Actin	180	82.0–82.5
	REV: TCACACGGCAGGCATACT
CCND1	FOR: CAATGACCCCGCACGATTTC	Actin	146	85.0
	REV: CATGGAGGGCGGATTGGAA
CCND2	FOR: ACCTTCCGCAGTGCTCCTA	Actin	161	85.5
	REV: CCCAGCCAAGAAACGGTCC
CSF1	FOR: AGACCTCGTGCCAAATTACATT	Actin	248	84.5–85.0
	REV: AGGTGTCTCATAGAAAGTTCGGA
FASN	FOR: ACAGCGGGGAATGGGTACT	RPII	136	88.0
	REV: GACTGGTACAACGAGCGGAT
GSTM3	FOR: TACCTCTTATGAGGAGAAACGGT	Actin, RPII	100	82.0–82.5
	REV: AGGAAAGTCCAGGTCTAGCTTG
MYC	FOR: GTCAAGAGGCGAACACACAAC	Actin	130	84.5–85.0
	REV: TTGGACGGACAGGATGTATGC
PANX1	FOR: GCTCTTTGCGATCCTCCTGTA	Actin	118	83.5
	REV: TGCACGGTTGTAAACTTTGTCAA
SCD	FOR: ACCGCTCTTACAAAGCTCGG	Actin	154	84.5
	REV: CCACGTCGGGAATTATGAGGAT
SLC1A1	FOR: TTCTAATGCGGATGCTGAAACT	Actin	146	81.0
	REV: CGCGCAGACCAATTTTTCC
SLC1A3	FOR: AGCAGGGAGTCCGTAAACG	Actin	120	80.0–80.5
	REV: AGCATTCCGAAACAGGTAACTTT
SLC7A11 (xCT)	FOR: CCTCTATTCGGACCCATTTAG	Actin	99	80.0–80.5
	REV: CTGGGTTTCTTGTCCCATATA
SLC25A1	FOR: TTCCCCACCGAGTACGTGAA	RPII	147	89.5–90.0
	REV: GTAGAGCAGGGAGCTAAGGC
SOCS3	FOR: CCTGCGCCTCAAGACCTTC	Actin	99	87.0
	REV: GTCACTGCGCTCCAGTAGAA
SUMO3	FOR: GAATGACCACATCAACCTGAAGG	RPII	152	87.5–88.0
	REV: GCCCGTCGAACCTGAATCT