Table 3. 18 DEGs identified by RNA-sequencing that were further validated by qPCR.
Cell Type | Gene | RNA-seq Fold Δ (q value) | qPCR Fold Δ (p value) |
---|---|---|---|
MDA-MB-231 WT vs. Clone #2 | ADIPOR1 | 1.59 (0.00036) | 1.70 (0.01) |
AKT1 | 0.78 (0.037) | 0.66 (0.04) | |
BCAR3 | 0.34 (0.00036) | 0.48 (0.03) | |
CCDN1 | 1.44 (0.00036) | 1.54 (0.002) | |
CCND2 | 2.94 (0.0036) | 2.50 (0.00002) | |
FASN | 0.56 (0.0013) | 0.59 (0.02) | |
GSTM3 | 0.58 (0.00036) | 0.52 (0.002) | |
MYC | 0.51 (0.00036) | 0.69 (0.009) | |
PANX1 | 2.05 (0.00036) | 2.44 (0.0002 | |
SCD | 0.32 (0.00036) | 0.48 (0.03) | |
SLC1A1 | 2.85 (0.00036) | 2.64 (0.0007) | |
SLC1A3 | 1.83 (0.0038) | 1.55 (0.006) | |
SLC25A1 | 0.73 (0.0007) | 0.34 (0.03) | |
SLC7A11 (xCT) | 0.56 (0.00036) | 0.69 (0.03) | |
SOCS3 | 0.80 (0.027) | 0.52 (0.0008) | |
SUMO3 | 0.84 (0.040) | 0.54 (0.02) | |
T47D WT vs. Clone #1 | BCAR3 | 2.00 (0.015) | 4.8 (0.002) |
B2M | 1.49 (0.039) | 1.43 (0.008) | |
CSF1 | 2.48 (0.019) | 5.93 (0.02) | |
GSTM3 | 1.98 (0.0022) | 2.84 (0.001) | |
MYC | 2.46 (0.0022) | 2.93 (0.005) | |
SLC7A11 (xCT) | 3.40 (0.0022) | 3.31 (0.02) |