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. 2016 Aug 11;11(8):e0161163. doi: 10.1371/journal.pone.0161163

Fig 2. Generation of KRT17-overexpressing and KRT17-knockdown oral cancer cell lines.

Fig 2

(A) Agarose gel electrophoresis of RT-PCR assays for the identification of KRT17 and GAPDH in various oral cancer cell lines. (B) Western blot analysis of various oral cancer cell lines for detection of KRT17 and GAPDH. (C) Northern blot analysis of Ca9 and HSC3 cells by hybridization with a KRT17 probe. (D) Immunofluorescent images showing filamentous staining of KRT17 in Ca9 (faint) and HSC3 cells. Scale bar, 10 μm. (E) Western blot analysis of KRT17-overexpressing Ca9 (Ca9/K17+) clones (C-1, C-2, C-3, and C-4), the parental Ca9 cells, KRT17-knockdown HSC3 (HSC3/K17-) clones (Kd-1, Kd-2, Kd-3, and Kd-4) and the parental HSC3 cells for detection of KRT17 and beta1-tubulin (TUBB). KRT17 cDNA was transfected into Ca9 to make KRT17-overexpressing cells (Ca9/K17+), and four independent clones (C-1, C-2, C-3, and C-4) were established. KRT17 expression in HSC3 was suppressed by miRNA-mediated knockdown, and four independent clones (Kd-1, Kd-2, Kd-3, and Kd-4) of KRT17-knockdown cells (HSC3/K17-) were established.

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