Fig 3. KRT17 promoted cell proliferation and migration.

(A) Cell proliferation assay after 72h of culture. C-4 showed a significantly higher rate of proliferation than the control. *P < 0.05 compared with the control. C-1, C-2, and C-3 showed a tendency towards elevated proliferation (0.05 < P < 0.1). HSC3/K17- showed significantly lower rates of proliferation than the control. Representative graphs of n = 3 independent experiments (each experiments comprised three technical replicates). **P < 0.01 and *P < 0.05 compared with the control. Data represent mean ± SEM. (B) Transwell migration assay using Boyden chambers. Cells (2 x 10⁵ cells/L) were suspended in serum-free medium and seeded into the upper chamber with pores of 8 μm. The lower chamber was filled with serum-containing medium. The upper chamber was confluent with cells during the assay. After 48 h, the cells that had migrated into the lower chamber through the filter were stained with crystal violet and counted in three microscopic fields per sample. Representative graphs of n = 3 independent experiments (each experiments comprised three technical replicates). **P < 0.01 compared with the control. Data represent mean ± SEM. (C) Transwell invasion assay. The filters were coated with an extracellular matrix protein mixture, and a transwell migration assay was conducted. Representative graphs of n = 3 independent experiments (each experiment comprised three technical replicates). **P < 0.01 compared with the control. Data represent mean ± SEM.