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. 2016 Apr 14;12(2):78–93. doi: 10.1080/15476278.2016.1172164

FIGURE 3.

FIGURE 3.

Elovl1 knockdown impairs swim bladder inflation. (A-E) 10 ng MOelovl1a or MOelovl1b alone, a half dose (5 ng each) of both MOs (*MOelovl1a+1b), or single MO + corresponding mRNA (50 pg elovl1a or 250 pg elovl1b) were injected at the one-cell stage of zebrafish embryos. Injection of either MOelovl1a or MOelovl1b, and a half dose of both MOs resulted in a deflated swim bladder, whereas co-injection of either MOelovl1a + elovl1a mRNA or MOelovl1b + elovl1b mRNA rescued the defective swim bladder phenotype. Embryos displaying the swim bladder inflation defect at 5 dpf are shown in lateral (B-D) or dorsal (B′-D′) views with the anterior aspect to the left. (F) The percentages of affected embryos (43% (n = 184) for MOelovl1a, 16% (n = 184) for MOelovl1a + elovl1a mRNA, 27% (n = 201) for MOelovl1b, 10% (n = 201) for MOelovl1b + elovl1b mRNA, and 40% (n = 265) for a half dose of combined *MOelovl1a+1b) with the number of embryos in each category are shown in a graph. The total number of embryos shown inside each bar was counted from at least 3 independent experiments for each condition. Arrow points the region where the swim bladder develop.