FIGURE 7.

Elovl1b knockdown affects genes required for kidney development. Embryos were injected with MOelovl1b and whole mount in situ hybridization was performed to analyze expression of genes involved in kidney development. Genes expressed in the kidney tubule were significantly reduced in elovl1b knockdown embryos (for each gene at 24 hpf, wt1b, 0%, n = 51; cdh17, 24%, n = 50; trpm7, 32%, n = 51; slc12a1, 24%, n = 54; slc12a3, 40%, n = 49; and at 60 hpf, wt1b, 0%, n = 48; cdh17, 28%, n = 50; trpm7, 28%, n = 53; slc12a1, 32%, n = 51; slc12a3, 32%, n = 52) as compared to those in the control. Embryos are presented in dorsal views (A – H) or lateral views (I – T) with the anterior aspect to the left.