Table 2. Summary of the QTLs associated with flowering time, maturity, plant height and grain yield on 158 recombinant inbred lines derived from a cross between spring wheat cultivars, ‘Cutler’ and ‘AC Barrie’.
The results are for the combined two environments for flowering time under greenhouse and five environments for flowering time, maturity, plant height and grain yield under field conditions.
| QTL | Trait* | Condition | Chrom | Position (cM) | Confidence interval (cM) | Left marker | Right marker | LOD | Partial R2 (%) | Additive effect** | Difference*** |
|---|---|---|---|---|---|---|---|---|---|---|---|
| QFlt.dms-2D | Flowering (days) | GH | 2D | 0 | 0–3 | Ppd-D1a | wsnp_CAP11_c3842_1829821 | 16.3 | 36.6 | -2.6 | -5.4 |
| QFlt.dms-5A.1 | Flowering (days) | GH | 5A | 187 | 183–199 | Kukri_c20258_143 | JD_c3525_1503 | 3.3 | 6.9 | 0.9 | 2.0 |
| QFlt.dms-5B | Flowering (days) | GH | 5B | 44 | 43–48 | BS00063785_51 | IACX5818 | 5.0 | 12.3 | -1.1 | -1.8 |
| QFlt.dms-6B.1 | Flowering (days) | GH | 6B | 59 | 56–60 | Tdurum_contig11700_1247 | wsnp_Ra_c2730_5190365 | 4.2 | 10.0 | -1.1 | -2.0 |
| QFlt.dms-7A | Flowering (days) | GH | 7A | 5 | 4–8 | Excalibur_c16355_712 | RAC875_c18446_521 | 3.7 | 7.3 | -1.0 | -1.4 |
| QFlt.dms-2D | Flowering (days) | Field | 2D | 0 | 0–5 | Ppd-D1a | wsnp_CAP11_c3842_1829821 | 7.2 | 19.6 | -0.8 | -1.1 |
| QFlt.dms-3B | Flowering (days) | Field | 3B | 94 | 91–95 | Excalibur_c45968_83 | CAP12_rep_c7901_114 | 5.8 | 13.5 | 0.6 | 1.0 |
| QFlt.dms-6B.2 | Flowering (days) | Field | 6B | 118 | 110–120 | wsnp_Ex_c4124_7455225 | Kukri_c49331_77 | 3.1 | 6.7 | -0.4 | -0.7 |
| QFlt.dms-7A | Flowering (days) | Field | 7A | 9 | 8–10 | Tdurum_contig11613_329 | wsnp_Ex_c30239_39179460 | 4.7 | 12.7 | -0.5 | -0.9 |
| QFlt.dms-2D | Flowering (DD) | Field | 2D | 0 | 0–5 | Ppd-D1a | wsnp_CAP11_c3842_1829821 | 7.8 | 25.4 | -14.2 | -21.0 |
| QFlt.dms-3B | Flowering (DD) | Field | 3B | 94 | 91–95 | Excalibur_c45968_83 | CAP12_rep_c7901_114 | 6.7 | 10.7 | 10.6 | 19.7 |
| QFlt.dms-4A | Flowering (DD) | Field | 4A | 41 | 38–42 | CAP12_rep_c4000_432 | wsnp_Ex_c54453_57331510 | 3.7 | 6.3 | -7.6 | -17.6 |
| QFlt.dms-5A.2 | Flowering (DD) | Field | 5A | 253 | 252–254 | Tdurum_contig86202_175 | wsnp_Ra_c10915_17838202 | 3.7 | 7.5 | 7.5 | 16.1 |
| QFlt.dms-6B.2 | Flowering (DD) | Field | 6B | 118 | 111–120 | wsnp_Ex_c4124_7455225 | Kukri_c49331_77 | 3.4 | 9.3 | -7.4 | -13.8 |
| QFlt.dms-7A | Flowering (DD) | Field | 7A | 9 | 8–10 | Tdurum_contig11613_329 | wsnp_Ex_c30239_39179460 | 6.4 | 15.6 | -10.3 | -15.9 |
| QMat.dms-2D | Maturity (days) | Field | 2D | 0 | 0–7 | Ppd-D1a | wsnp_CAP11_c3842_1829821 | 3.1 | 11.2 | -0.7 | -1.3 |
| QMat.dms-4A.2 | Maturity (days) | Field | 4A | 53 | 51–56 | Ra_c7973_1185 | wsnp_Ex_c10390_17007929 | 3.5 | 6.5 | -0.7 | -1.2 |
| QMat.dms-4D.1 | Maturity (days) | Field | 4D | 26 | 19–29 | Excalibur_c5010_1336 | Kukri_rep_c68594_530 | 4.9 | 13.4 | 0.8 | 1.6 |
| QMat.dms-7A.2 | Maturity (days) | Field | 7A | 44 | 42–45 | RAC875_c14982_577 | Tdurum_contig20214_279 | 3.1 | 8.8 | 0.6 | 1.2 |
| QMat.dms-2D | Maturity (DD) | Field | 2D | 0 | 0–8 | Ppd-D1a | wsnp_CAP11_c3842_1829821 | 3.9 | 10.4 | -14.2 | -21.2 |
| QMat.dms-4A.1 | Maturity (DD) | Field | 4A | 41 | 35–42 | CAP12_rep_c4000_432 | wsnp_Ex_c54453_57331510 | 3.1 | 12.0 | -10.7 | -22.7 |
| QMat.dms-4D.2 | Maturity (DD) | Field | 4D | 37 | 34–43 | Rht-D1b | wsnp_CAP11_c356_280910 | 5.7 | 16.0 | 15.7 | 33.0 |
| QMat.dms-7A.1 | Maturity (DD) | Field | 7A | 13 | 10–16 | wsnp_Ra_c63822_63288359 | wsnp_BG313770A_Ta_2_3 | 3.0 | 9.1 | -11.3 | -11.2 |
| QMat.dms-7A.2 | Maturity (DD) | Field | 7A | 42 | 40–45 | Tdurum_contig37154_190 | RAC875_c14982_577 | 5.7 | 16.2 | 15.7 | 20.8 |
| QPht.dms-4D | Plant height | Field | 4D | 37 | 35–39 | Rht-D1b | wsnp_CAP11_c356_280910 | 16.2 | 37.8 | -6.3 | -13.2 |
| QYld.dms-2D | Grain yield | Field | 2D | 2 | 0–12 | Ppd-D1a | wsnp_CAP11_c3842_1829821 | 4.3 | 8.6 | -248.2 | -436.0 |
| QYld.dms-5B | Grain yield | Field | 5B | 34 | 32–36 | Excalibur_c30667_102 | Ku_c6193_821 | 3.7 | 7.7 | -184.0 | -321.8 |
* Flowering (days) = number of days to 50% flowering under greenhouse (GH) or field conditions; Maturity (days) = number of days to maturity under field condition; Flowering (DD) = flowering time in degree days; Maturity (DD) = maturity in degree days.
** Additive effect is half the difference between the genotypic values of the two parents. For grain yield, negative additive effects indicate that the favorable alleles originated from ‘AC Barrie’. For flowering, maturity and plant height, negative additive effects indicate the opposite (the favorable alleles originated from ‘Cutler’).
***Difference = the difference in phenotypic values of all RILs that had the ‘Cutler’ alleles at both flanking markers of every QTL to those that had the ‘AC Barrie’ alleles. The units for differences are number of days or degree days for both flowering and maturity, cm for plant height, and kg ha-1 for grain yield.