Fig 1. Experimental design.

Beginning at age 6 wk, NZBWF1 were assigned CON diet or 0.4, 1.2, and 2.4% DHA diet. NZW/LacJ mice were assigned either CON diet or 2.4% DHA-containing diet. Then, starting at age 8 wk, mice were dosed intranasally with 25 μl PBS VEH or 25 μl PBS containing 1.0 mg cSiO₂ weekly for 4 wk. Proteinuria was monitored over the course of the experiment and all animals euthanized at 23 wk of age (i.e. 12 wk PI).