Fig 11. cSiO₂ -induced elevations of proinflammatory cytokines MCP-1, TNF-α and IL-6 in BALF and plasma are decreased by DHA consumption in NZBWF1 mice.

MCP-1 (A, D), TNF-α, (B, E) and IL-6 (C, F)) were quantitated in BALF (left panel) and plasma (right panel) by flow cytometric bead array. Data are $\overline{\mathrm{x}}$ ± SEM (n = 8). The designation n.d. indicates below the limit of detection. Bars without same letter are significantly different (p<0.05). Symbols: * indicates significant difference from CON-fed mice instilled with VEH (p < 0.05); # indicates significant difference from CON-fed mice instilled with cSiO₂ (p < 0.05). DHA dose-dependently decreased BALF concentrations of MCP-1 (r² = -0.791, p < 0.001), TNF- α (r² = -0.577 p < 0.001), and IL-6 (r² = -0.810, p < 0.001). DHA dose-dependently decreased plasma MCP-1 (r² = -0.871, p < 0.001) and TNF-α (r² = -0.527, p < 0.05).